Dideoxy termination method

Sequencing of HBV ( adr ) DNA Restrictive Fragment by Dideoxy Chain Termination Method

末端终止法测定HBV（adr亚型）DNA酶解片断序列

Dideoxy chain termination method was applied to sequence hepatitis E virus structure gene of Asia isolates , cDNA sequence and amino acid residues were determined .

目的了解戊型肝炎病毒的变异。方法用双脱氧法对亚洲主要戊型肝炎流行国家的戊型肝炎病毒结构基因区段进行了分析，并根据cDNA序列确定了其氨基酸的序列。

The positive recombinant plasmid was identified by PCR , enzyme digestion . The nucleotide sequence of CSP3 ′ ending gene was determined by the dideoxy chain termination method .

阳性重组质粒经PCR、酶切鉴定，用双脱氧链末端终止法进行序列测定。

Methods The LDH gene was amplified by PCR technique from genomic DNA of isolate FCC 1 / HN . Then it was cloned into pMD-18T vector . The cloned LDH gene was deter - mined by the dideoxy chain termination method .

方法根据LDH基困已知序列设计合成一对引物，应用PCR技术从FCC1／HN株基因组DNA中扩增LDH基因，并将其克隆入pMD18－T载体。

The M RNA segment of Hantaan virus Chinese isolate A9 was reverse-transcribed into the first strand of cDNA and amplified into cDNA fragments which were cloned into pGEM-T vector . The positive clones were sequenced on double strand level by dideoxy chain termination method .

采用反转录-PCR方法，分节段扩增汉坦病毒A9株M基因片段cDNA，并克隆入PGEMT载体中，用双脱氧末端终止法直接测定序列。

The amplified product with 238 bp was purified and then directly sequenced by dideoxy nucleotide chain termination method .

PCR产物纯化后直接经双脱氧链末端终止法测定核苷酸序列。

In addition , Sanger method ( dideoxy mediated chain termination method ) of DNA sequencing was used to examine the exon 7 which was positive in p53 mutation .

运用双脱氧链终止法对第7外显子阳性突变标本进行DNA测序。